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Heinz Wolff building, Brunel University, Kingston Ln, London, Uxbridge

* * This course contributes to the Royal Society of Biology's Technical Skills Certificate

* * A discount is available to those who book both this couse and our Microscopy and Microbiology Practical. This discount is £320 for both courses for members and £640 for both courses for non-members

Bookings close on the 26th August at 5pm.
Please note, you will need to arrive at the lab in the Heinz Wolff building at 09:45, for a 10:00 start.


The world is full of pathogens that infect us and cause disease. Often these can be grown in culture for identification purposes, but with some pathogens it is difficult to collect samples, they are difficult to grow and diagnosis can take a long time. Therefore, other diagnostic techniques must be used in clinical setting to allow rapid and cost-effective detection and identification. One such technique routinely used is the enzyme-linked immunosorbent assay (ELISA). This is an immunological based assay that can be used to measure antibodies, antigens, proteins and glycoproteins associated with the pathogen in biological samples. On this practical course you will learn about the different types of ELISA, how they work, and use a working example to detect if some patients have an immune response to Aspergillus fumigatus.

You will learn both the Indirect and Sandwich ELISA techniques, understand the pathogen produced by Aspergillus fumigatus, and how it can induce a hyperactive immune response (allergic response) to the presence of this pathogen.


The broad aims of the course are to:
•    Provide the foundations of what ELISA is and why it is important for patient diagnosis.
•    Introduce the equipment and techniques associated with undertaking different types of ELISA.
•    Provide practical application of undertaking ELSA tests.
•    Guide on the analysis of results and what the results mean.

Who is the Course For?

Biological and medical scientists who wish to learn the ELISA technique, and how to refine and apply it. No prior knowledge of the ELISA technique will be required, and the purpose of the course is to offer a practical point of access to this technique in a practical user-friendly form.

Learning Outcomes

Knowledge and understanding:
•    Understand the principles of the enzyme-linked immunosorbent assay (ELISA).
•    Learn about Aspergillus fumigatus and how it can cause both infections and allergic responses.
•    Learn the different types of ELISA (Indirect and Sandwich).
•    The practical applications of ELISA.
•    Understand how the data is analysed and what does it mean.

Course Tutor

The course will be run by Dr Ashley Houlden. Ashley is a microbial ecologist, with research interests in microbial community (host-microbiome) structure and function. He has studied soil microbial ecology relating to fungi diseases in crops, before moving into research on the medical microbiome.
Ashley is a co-investigator in the “Organ-on-a-Chip” interdisciplinary research group at Brunel University London. This research group focuses on developing in vitro models of the breast, vagina, ovary and placenta, with a view to their use in pre-clinical trials of new drugs.

Ashley is currently a Lecturer in the Division of Biosciences, College of Health, Medical and Life Sciences at Brunel University London. He teaches at second year undergraduate and at MSc level, and has also been involved in providing lab “taster” sessions for secondary school children and our BSc Biomedical Science course applicants.

Certification and Continuing Professional Development (CPD)

A certificate of attendance will be provided after the event.
We evaluate all of our training events, to make sure that we maintain a high quality of training. This event has been approved by the Royal Society of Biology for purposes of CPD and can be counted as 9 CPD points.

Professional Registers

This course supports registers competencies and has been identified as supporting competency development for: Registered Science Technician (RSciTech).


Members - £150 + VAT
Members of Member Organisations, SCAS members - £225 + VAT
Non-members - £300 + VAT
Non-members who have completed a membership application and made payment can also benefit from the discounted member rate.

If you or your organisation wishes to book staff in bulk for this course, please get in touch. 


For further information about the course please contact Francesca Chantry, training and registers officer at or on 020 3925 3457.


Unfortunately, the Royal Society of Biology is unable to offer refunds on training courses that have been attended. We do, of course, welcome and encourage any feedback from a course and will continue to improve the service we offer.

Terms and Conditions

By booking to attend this event, you are confirming you have agreed to the RSB's Terms and Conditions which can be found here.