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15.05.23 - 14.06.24
BioGrad education, Liverpool Science Park, 131 Mount Pleasant, Liverpool L3 5TF


Mammalian Cell Culture is a highly sought-after lab technique that is often requested for graduate-level researcher positions in academia and industry. Our Mammalian Cell Culture course offers comprehensive laboratory-based tuition using a range of adherent & suspension cell lines and primary cells, thus providing a rare opportunity to gain hands on experience that is crucial for future careers in Biology, Biomedical Science, Pharmacology & Medical Research.

This course is designed to introduce students to the fundamental concepts and techniques of mammalian cell culture to help them carry out core cell culture techniques properly and safely. The course runs for 5 days of lab-based tuition and a total of 35 hours of delivery. Working in small groups, students receive comprehensive tuition with experienced scientists covering the laboratory requirements and legal regulations of cell culture in the UK.

There are a variety of dates available to undertake this 5-day course, from May 2023 - June 2024. Please visit the BioGrad education event page for further detail.

What the course will cover:

• All aspects of Good Cell Culture Practice (GCCP)- Aseptic technique and contamination control
• Sterile media preparation
• Passaging of suspension and adherent cell lines
• Cryopreservation and resuspension of cell stocks
• Analysis of confluency, morphology and viability
• Cell counting & density calculations
• Isolation of peripheral blood mononuclear cells (PBMCs) using density centrifugation
• Cellular differentiation
• Histological staining, brightfield & inverted microscopy
• Cell viability assay (commonly used for in vitro drug predictions and nanotoxicology research).

The laboratory results obtained by the students are analysed and discussed. Strong focus is set on the student acquiring a good sterilisation technique through practical training and supervision.

Attendance Requirements

Students attending this course are expected to have previous laboratory experience, therefore, a questionnaire must be completed prior to the commencement of your course to ensure you are able to meet the required competencies.

If you do not yet possess the necessary lab skills, you need to attend the BioGrad 1 day ‘Introduction to laboratory skills' course or the 5 days ‘Essential Laboratory Techniques' course before commencing this course.

Further information and booking

For more information and to book please see the Biograd Training event webpage.

Continuing Professional Development (CPD)

This event has been approved by the Royal Society of Biology for the purposes of CPD and counts as 90 CPD points.