Mammalian Cell Culture is a highly sought-after lab technique that is often requested for graduate-level researcher positions in academia and industry. Our Mammalian Cell Culture course offers comprehensive laboratory-based tuition using a range of adherent & suspension cell lines and primary cells, thus providing a rare opportunity to gain hands on experience that is crucial for future careers in Biology, Biomedical Science, Pharmacology & Medical Research.

• All aspects of Good Cell Culture Practice (GCCP)- Aseptic technique and contamination control

• Sterile media preparation

• Passaging of suspension and adherent cell lines

• Cryopreservation and resuspension of cell stocks

• Analysis of confluency, morphology and viability

• Cell counting & density calculations

• Isolation of peripheral blood mononuclear cells (PBMCs) using density centrifugation

• Cellular differentiation

• Histological staining, brightfield & inverted microscopy

• Cell viability assay (commonly used for in vitro drug predictions and nanotoxicology research).

The laboratory results obtained by the students are analysed and discussed. Strong focus is set on the student acquiring a good sterilisation technique through practical training and supervision.

For more information and to book please see the Biograd Training event webpage.